abstract：:Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

journal_title：Microbiology and immunology

authors： Kawana T,Hashido M,Koizumi Y

更新日期：1995-01-01 00:00:00

abstract：:To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhim...

journal_title：Microbiology and immunology

authors： Murase T,Okitsu T,Suzuki R,Morozumi H,Matsushima A,Nakamura A,Yamai S

更新日期：1995-01-01 00:00:00

abstract：:The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP)...

journal_title：Microbiology and immunology

authors： Nishimura H,Sugawara K,Gao P,Muraki Y,Hongo S,Kitame F,Nakamura K

更新日期：1995-01-01 00:00:00

abstract：:Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to...

journal_title：Microbiology and immunology

authors： Saruta K,Hoshina S,Machida K

更新日期：1995-01-01 00:00:00

abstract：:Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on sixteen human isolates of serotype 2 of rotavirus in Japan, China, and Pakistan and their genetic variations were examined. Comparative studies of their nucleotide and deduced amino acid sequences between the sixteen is...

journal_title：Microbiology and immunology

authors： Wen L,Ushijima H,Kakizawa J,Fang ZY,Nishio O,Morikawa S,Motohiro T

更新日期：1995-01-01 00:00:00

abstract：:A polymerase chain reaction (PCR) method was performed for rapid and sensitive detection of pathogenic Vibrio trachuri isolated from cultured Japanese horse mackerel. A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specif...

journal_title：Microbiology and immunology

authors： Iwamoto Y,Suzuki Y,Kurita A,Watanabe Y,Shimizu T,Ohgami H,Yanagihara Y

更新日期：1995-01-01 00:00:00

abstract：:Previously, we have reported the occurrence of novel phosphocholine-containing glycoglycerolipids (GGPLs: GGPL-I and GGPL-III) in human helper T-cell (MT-4 cell line) (Mustuda et al, Glycoconjugate J. 10:340). However, the GGPLs disappeared from the MT-4 after treatment with an antimycoplasma agent. This disappearance...

journal_title：Microbiology and immunology

authors： Matsuda K,Harasawa R,Li JL,Kasama T,Taki T,Handa S,Yamamoto N

更新日期：1995-01-01 00:00:00

abstract：:Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of...

journal_title：Microbiology and immunology

authors： Hotsubo T,Nagata N,Shimada M,Yoshida K,Fujinaga K,Chiba S

更新日期：1994-01-01 00:00:00

abstract：:Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a de...

journal_title：Microbiology and immunology

authors： Kim EH,Aoki T

更新日期：1994-01-01 00:00:00

abstract：:The function of MHC class II HLA-DR molecules expressed on a human B lymphoid progenitor cell line FL8.2.4.4 (abbreviated as FL4.4) was examined. FL4.4 cells expressed HLA-DR molecules and stimulation of the DR molecules by anti-DR mAb or by superantigen TSST-1 induced strong augmentation of homocytic aggregation and ...

journal_title：Microbiology and immunology

authors： Naitoh K,Ichigi Y,Miyake K,Muraguchi A,Kimoto M

更新日期：1994-01-01 00:00:00

abstract：:The genospecies Borrelia afzelii was isolated from a patient of Lyme disease in Hokkaido, Japan, for the first time, by culturing the minced erythema lesion in BSK II medium. Two analytical methods, rRNA gene restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) using the specific primer ...

journal_title：Microbiology and immunology

authors： Nakao M,Miyamoto K,Fukunaga M,Hashimoto Y,Takahashi H

更新日期：1994-01-01 00:00:00

abstract：:Although the RC-HL strain of rabies virus is avirulent in adult mice, the amino acid at position 333 of its G protein is arginine, which is thought to be necessary for virulence in adult mice upon intracerebral inoculation of the virus. This result suggests that besides arginine at position 333, some other positions o...

journal_title：Microbiology and immunology

authors： Ito H,Minamoto N,Watanabe T,Goto H,Rong LT,Sugiyama M,Kinjo T,Mannen K,Mifune K,Konobe T

更新日期：1994-01-01 00:00:00

abstract：:Porins were prepared from smooth strain of Salmonella typhi 0-901 and chemotype rough mutant of S. typhimurium Ra-30. Porins could significantly stimulate the immune systems of mice. Immunization of mice with the porins provoked synthesis of anti-porin antibodies. Macrophages from the immunized mice showed increased c...

journal_title：Microbiology and immunology

authors： Tabaraie B,Sharma BK,Sharma PR,Sehgal R,Ganguly NK

更新日期：1994-01-01 00:00:00

abstract：:Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/I...

journal_title：Microbiology and immunology

authors： Guth BE,Aguiar EG,Griffin PM,Ramos SR,Gomes TA

更新日期：1994-01-01 00:00:00

abstract：:L forms of Salmonella typhimurium LT2 conferred strong protection to a lethal challenge with its parental bacterium on innately hypersusceptible C3H/HeJ mice, and its minimal protective dose was approximately 150 L-forming units. Although L-form S. typhimurium was avirulent for C3H/HeJ mice, it multiplied slowly in bo...

journal_title：Microbiology and immunology

authors： Nishikawa F,Kita E,Yamada H,Nakano A,Kashiba S

更新日期：1994-01-01 00:00:00

abstract：:The plasma concentration of soluble P-selectin (GMP-140/CD62P/PADGEM), a selectin produced by activated platelets and endothelial cells, was quantitated in a group of adults and East African negro children presenting with either non-severe or severe (cerebral) malaria caused by Plasmodium falciparum. Sixty percent of ...

journal_title：Microbiology and immunology

authors： Facer CA,Theodoridou A

更新日期：1994-01-01 00:00:00

abstract：:A polymyxin B (PXB)-resistant mutant of Klebsiella pneumoniae O3 was isolated. Lipopolysaccharide (LPS) extracted from the PXB-resistant isolate bound little PXB, although LPS from the parental strain did. The 31P nuclear magnetic resonance (NMR) spectrum of PXB-resistant type LPS showed that it contained much less of...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Jiang GZ,Kato Y,Sugiyama T,Kawai M,Fukada M,Takahashi K

更新日期：1994-01-01 00:00:00

abstract：:Staphylococcus aureus strains isolated from bovine intramammary infection (mastitis) were tested for adhesion to bovine mammary epithelial cells after growth in milk whey or TSB. Bacteria grown in milk whey adhered more efficiently to mammary gland epithelial cells in vitro than the corresponding homologous bacteria g...

journal_title：Microbiology and immunology

authors： Mamo W,Fröman G

更新日期：1994-01-01 00:00:00

abstract：:Antibodies to fibronectin-binding proteins (FnBPs) of Staphylococcus aureus, including binding domain of FnBPA, the D region, or the A-C regions of FnBPB were produced in rabbits and mice. These antibodies were used to characterize cell-associated FnBPs of S. aureus strain Cowan I, S. aureus strain U320 and a coagulas...

journal_title：Microbiology and immunology

authors： Sakata N,Rozalska B,Wadström T

更新日期：1994-01-01 00:00:00

abstract：:Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of...

journal_title：Microbiology and immunology

authors： Hamaoka T,Terakado N,Nakamura S

更新日期：1994-01-01 00:00:00

abstract：:The polymerase chain reaction (PCR) was applied for the etiological diagnosis of spotted fever group (SFG) rickettsiosis in Japan. Nucleotide primers derived from the 17-kDa antigen gene of Rickettsia rickettsii primed a rickettsia-specific 246-base-pair product for all of the Katayama, Abe, Misaka and Kojima strains,...

journal_title：Microbiology and immunology

authors： Tange Y,Matsumoto M,Okada T,Hasegawa H,Kobayashi Y

更新日期：1994-01-01 00:00:00

abstract：:A chemotaxonomic study was carried out with a new serotyping scheme comprising 35 O-antigen groups of Vibrio fluvialis on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharide (LPS). A previously developed rapid method of preparing samples for compositional sugar analysis was emp...

journal_title：Microbiology and immunology

authors： Iguchi T,Kondo S,Hisatsune K

更新日期：1993-01-01 00:00:00

abstract：:Cytochalasin B (CB) and cytochalasin D (CD), inhibitors of microfilament function of host cell, were examined for their effects on Staphylococcus aureus Cowan I adherence to and ingestion by several types of the hyperosmolarity-tolerant (HOT) cells obtained from primary culture of mouse kidney. Staphylococcal adherenc...

journal_title：Microbiology and immunology

authors： Murai M,Seki K,Sakurada J,Usui A,Masuda S

更新日期：1993-01-01 00:00:00

abstract：:Although chronic fatigue syndrome (CFS) is known to be the syndrome that begins with an acute flu-like illness that may be due to the exposure to an infectious agent, there has been no convincing evidence on the causative agents. Recently, human T-lymphotropic virus type II (HTLV-II)-like virus has been reported to be...

journal_title：Microbiology and immunology

authors： Honda M,Kitamura K,Nakasone T,Fukushima Y,Matsuda S,Nishioka K,Matsuda J,Hashimoto N,Yamazaki S

更新日期：1993-01-01 00:00:00

abstract：:Borrelia duttonii strain 406K, a causative agent of relapsing fever, could not be cultivated in vitro in currently available media for borreliae. We have developed an in vitro cultivation system by using SflEp cell cultures. The average increases of the number of borreliae, when inoculated with 1.0 x 10(5) organisms p...

journal_title：Microbiology and immunology

authors： Konishi H,Morshed MG,Akitomi H,Nakazawa T

更新日期：1993-01-01 00:00:00

abstract：:TtT/M-87 cell is a macrophage cell line established from thyrotropic pituitary tumor tissues in mouse. In this paper, we report the immunological properties of M-87 cells as a model of tumor-associated macrophage. Contrasting with resident peritoneal macrophages, M-87 cells constitutively secreted small but significan...

journal_title：Microbiology and immunology

authors： Mitsuishi T,Akuzawa Y,Sato S,Rui J,Kodama K,Inoue K,Kurashige S

更新日期：1993-01-01 00:00:00

abstract：:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...

journal_title：Microbiology and immunology

authors： Nair GB,Bhattacharya SK,Takeda T

更新日期：1993-01-01 00:00:00

abstract：:The slime-producing Staphylococcus epidermidis strain sensu strictu CNS23 was transformed by protoplast transformation with the plasmid pTV1 which carries transposon Tn 917. Using this transposon mutagenesis system we obtained the Tn917-inserted mutant CT512, which has lost the ability to produce slime. A single inser...

journal_title：Microbiology and immunology

authors： Grüter L,Feucht H,Mempel M,Laufs R

更新日期：1993-01-01 00:00:00

abstract：:Capture enzyme-linked immunosorbent assays (ELISAs) were developed to detect immunoglobulin G and M antibodies to group A streptococcal (GAS) antigens, streptolysin O, streptokinase, and group A carbohydrate. The sensitivities and the specificities of the IgM capture ELISAs to each GAS antigen were high enough to dist...

journal_title：Microbiology and immunology

authors： Hayashi S,Yokota K,Takizawa Y,Tomizawa I,Nejime T,Oguma K

更新日期：1993-01-01 00:00:00

abstract：:The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infec...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Masuda T,Kuroda MJ,Harada S

更新日期：1993-01-01 00:00:00

abstract：:We have previously identified a heterodimer molecule, Z-1, on guinea pig peritoneal macrophages (M phi s) by the newly prepared monoclonal antibody, anti-Z-1, and Z-1 has been assumed to be the complement receptor type three (CR3) in this species. To clarify this assumption, the cell type distribution of the antigen i...

journal_title：Microbiology and immunology

authors： Tamoto K,Nochi H,Tada M,Kakinuma T,Mori Y

更新日期：1993-01-01 00:00:00

abstract：:Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36...

journal_title：Microbiology and immunology

authors： Yoo YC,Yoshimatsu K,Yoshida R,Tamura M,Azuma I,Arikawa J

更新日期：1993-01-01 00:00:00

abstract：:We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the V...

journal_title：Microbiology and immunology

authors： Lin Z,Yamasaki S,Kurazono H,Ohmura M,Karasawa T,Inoue T,Sakamoto S,Suganami T,Takeoka T,Taniguchi Y

更新日期：1993-01-01 00:00:00

abstract：:The activities of a water-soluble peptidoglycan fragment derived from Staphylococcus epidermidis (SEPS) were examined as to their role in proliferation of spleen mononuclear cells (SMNC) from various strains of mice, the production of cytokines in vitro, and the induction of an inflammatory reaction in vivo. The proli...

journal_title：Microbiology and immunology

authors： Onta T,Sashida M,Fujii N,Sugawara S,Rikiishi H,Kumagai K

更新日期：1993-01-01 00:00:00

abstract：:The phospholipase C (alpha-toxin) gene (plc) of Clostridium perfringens was cloned into pUC19 and the effects of the upstream regions on expression of the plc gene were examined in Escherichia coli JM109. When the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase...

journal_title：Microbiology and immunology

authors： Toyonaga T,Matsushita O,Katayama S,Minami J,Okabe A

更新日期：1992-01-01 00:00:00

abstract：:Healthy cattle and swine bred in a district of Japan were examined for the presence of Clostridium botulinum in their liver. Liver specimens were cultivated in chopped meat-glucose medium and the cultures were examined for botulinum toxin. In cattle, none of the cultures of 100 liver specimens yielded the toxin. In sw...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Yoshimura K,Nakamura S

更新日期：1992-01-01 00:00:00

abstract：:The nucleic acid fraction from cells of 6 species of bacterium and 2 kinds of vertebrate, calf and salmon, was extracted and purified by the same procedures as described previously. When the spleen cells from BALB/c mice were incubated with the nucleic acid fraction from either of the bacteria, natural killer (NK) act...

journal_title：Microbiology and immunology

authors： Yamamoto S,Yamamoto T,Shimada S,Kuramoto E,Yano O,Kataoka T,Tokunaga T

更新日期：1992-01-01 00:00:00

abstract：:We prepared polyclonal antibody specific to Mycoplasma pneumoniae. Using this antibody, we developed a latex agglutination test (LAT) for detecting the organism in respiratory exudates as rapid diagnosis of M. pneumoniae infection. Further, LAT was compared with DNA-probe test (DP) which was the only commercially avai...

journal_title：Microbiology and immunology

authors： Masayoshi T,Hirai Y,Kanemasa Y

更新日期：1992-01-01 00:00:00

abstract：:Attempts were made to study the pathogenicity of some strains of Bacteroides fragilis group in the rat intra-abdominal abscess model. Multiple intraabdominal abscesses were produced in 50 to 70% of animals when an inoculum containing 10(9) CFU/ml of any of the five species of Bacteroides fragilis group was injected. R...

journal_title：Microbiology and immunology

authors： Jotwani R,Gupta U,Watanabe K,Ueno K

更新日期：1992-01-01 00:00:00

abstract：:Induction of interferon for a kind of dairy lactic acid bacteria, Lactobacillus acidophilus (L. acidophilus), was investigated in murine peritoneal macrophage (M phi) cultures. Lactobacillus acidophilus JCM 1034, 1132T, 1229 and 2125 induced IFN (12-34 I.U./ml) in M phi cultures in vitro. Strain 1132T- and 2125-induce...

journal_title：Microbiology and immunology

authors： Kitazawa H,Matsumura K,Itoh T,Yamaguchi T

更新日期：1992-01-01 00:00:00